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Role of the disulfide bond on the structure and activity of μ-conotoxin PIIIA in the inhibition of NaV1.4

Author:
Xu, Xiaoxiao, Xu, Qingliang, Chen, Fangling, Shi, Juan, Liu, Yuntian, Chu, Yanyan, Wan, Shengbiao, Jiang, Tao, Yu, Rilei
Source:
RSC advances 2019 v.9 no.2 pp. 668-674
ISSN:
2046-2069
Subject:
Conus purpurascens, binding capacity, disulfide bonds, models, skeletal muscle, sodium channels
Abstract:
μ-Conotoxin PIIIA, a peptide toxin isolated from Conus purpurascens, blocks the skeletal muscle voltage-gated sodium channel NaV1.4 with significant potency. PIIIA has three disulfide bonds, which contribute largely to its highly constrained and stable structure. In this study, a combination of experimental studies and computational modeling were performed to assess the effects of deletion of the disulfide bonds on the structure and activity of PIIIA. The final results indicate that the three disulfide bonds of PIIIA are required to produce the effective inhibition of NaV1.4, and the removal of any one of the disulfide bonds significantly reduces its binding affinity owing to secondary structure variation, among which the Cys11–Cys22 is the most important for sustaining the structure and activity of PIIIA.
Agid:
6269469