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One step DNA amplification of mammalian cells in picoliter microwell arrays

Author:
Liu, Wenwen, Li, Zhao, Liu, Yuanjie, Wei, Qingquan, Liu, Yong, Ren, Lufeng, Wang, Chenyu, Yu, Yude
Source:
RSC advances 2019 v.9 no.5 pp. 2865-2869
ISSN:
2046-2069
Subject:
DNA, mammals, silica
Abstract:
We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30 600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy.
Agid:
6269477