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Nano-Magnesium Oxide: A Novel Bactericide Against Copper-Tolerant Xanthomonas perforans Causing Tomato Bacterial Spot

Author:
Liao, Y.-Y., Strayer-Scherer, A. L., White, J., Mukherjee, A., De La Torre-Roche, R., Ritchie, L., Colee, J., Vallad, G. E., Freeman, J. H., Jones, J. B., Paret, M. L.
Source:
Phytopathology 2019 v.109 no.1 pp. 52-62
ISSN:
0031-949X
Subject:
Xanthomonas perforans, antibacterial properties, antibiotics, calcium, copper, copper nanoparticles, disease control, disease severity, field experimentation, fruits, greenhouse experimentation, in vitro studies, magnesium, magnesium oxide, manganese, mass spectrometry, minimum inhibitory concentration, potassium, tomatoes, transmission electron microscopy, Florida
Abstract:
Bacterial spot caused by Xanthomonas perforans causes significant damage on tomato in Florida. Due to the presence of copper (Cu)-tolerant X. perforans strains, Cu bactericides are not effective in disease management. Hence, there is a critical need to find alternatives for Cu. Antibacterial activity of magnesium oxide (Nano-MgO), and other metal oxide nanoparticles, were evaluated against a Cu-tolerant and -sensitive X. perforans strain. In vitro experiments demonstrated high antibacterial activity of Nano-MgO against both strains compared with the commercial Cu. The minimum inhibitory concentration of Nano-MgO is 25 µg/ml and the minimum bactericidal concentration is 100 µg/ml against a Cu-tolerant X. perforans strain after 4 h of exposure. Structural changes in the bacterial membrane following exposure to Nano-MgO treatments compared with the controls were observed using transmission electron microscopy. In two greenhouse experiments with a Cu-tolerant strain, bacterial spot severity was significantly reduced by Nano-MgO at 200 µg/ml compared with Cu-ethylene bis-dithiocarbamate (grower standard), and the untreated control (P = 0.05). In three field experiments, Nano-MgO at 200 µg/ml significantly reduced disease severity with no negative impact on yield compared with the untreated control. Inductively coupled plasma mass spectrometric analysis of the fruit confirmed that Nano-MgO application did not lead to the accumulation of Mg, Cu, Ca, K, Mn, P, and S. This study is the first to demonstrate the potential of Nano-MgO against bacterial spot of tomato.
Agid:
6270166