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Design of α-Keto Carboxylic Acid Dimers by Domain Recombination of Nonribosomal Peptide Synthetase (NRPS)-Like Enzymes
- Hühner, Elisabeth, Öqvist, Kristin, Li, Shu-Ming
- Organic letters 2019 v.21 no.2 pp. 498-502
- carboxylic acids, chemical structure, dimerization, enzymes, lactones, quinones
- Nonribosomal peptide synthetase (NRPS)-like enzymes comprising A–T–TE architectures catalyze the dimerization of α-keto carboxylic acids leading to the formation of hydroxybenzoquinones or lactones. Domain change experiments with five enzymes revealed that A and A–T domains of phenyl or 4-hydroxyphenyl pyruvate-using enzymes can be effectively used by the TE domains of other enzymes. Even the A and A–T domains of an indolyl hydroxybenzoquinone synthase were successfully recombined with TE domains of a phenyl and a 4-hydroxyphenyl pyruvate-activating enzyme.