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Winogradskyella profunda sp. nov. isolated from the Chukchi Sea bottom sediments

Author:
Romanenko, Lyudmila A., Kurilenko, Valeriya V., Guzev, Konstantin V., Svetashev, Vassilii I., Mikhailov, Valery V.
Source:
Archives of microbiology 2019 v.201 no.1 pp. 45-50
ISSN:
0302-8933
Subject:
DNA, bacteria, fatty acids, menaquinones, new species, phenotype, phosphatidylethanolamines, phylogeny, ribosomal RNA, sediments, sequence analysis, sodium chloride, Arctic region, Chukchi Sea
Abstract:
An aerobic, Gram-negative, yellow-pigmented non-motile rod-shaped bacterium, designated Ch38ᵀ, was isolated from a sediment sample collected from the Chukchi Sea in the Arctic Ocean. Comparative 16S rRNA gene sequence analysis positioned strain Ch38ᵀ into the genus Winogradskyella as a distinct line adjacent to Winogradskyella multivorans KCTC 23891ᵀ, sharing the highest similarities of 97.5%, 97.2%, and 97.1% with Winogradskyella eximia KCTC 12219ᵀ, Winogradskyella damuponensis KCTC 23552ᵀ, and Winogradskyella multivorans KCTC 23891ᵀ, respectively. Strain Ch38ᵀ grew at 5–36 °C and in the presence of 1–6% (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C₁₆:₀ 3-OH, anteiso-C₁₅:₀ followed by iso-C₁₅:₀ and iso-C₁₆:₁ as the major fatty acids. Polar lipids consisted of phosphatidylethanolamine, three unknown aminolipids, an unknown lipid and an unknown phospholipid. The DNA C + C content was 31.7 mol%. Based on the phylogenetic analysis and distinctive phenotypic characteristics, strain Ch38ᵀ is concluded to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella profunda sp. nov. is proposed. The type strain of the species is strain Ch38ᵀ (= KMM 9725ᵀ = KACC 19710ᵀ).
Agid:
6272424