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Winogradskyella profunda sp. nov. isolated from the Chukchi Sea bottom sediments

Romanenko, Lyudmila A., Kurilenko, Valeriya V., Guzev, Konstantin V., Svetashev, Vassilii I., Mikhailov, Valery V.
Archives of microbiology 2019 v.201 no.1 pp. 45-50
DNA, bacteria, fatty acids, menaquinones, new species, phenotype, phosphatidylethanolamines, phylogeny, ribosomal RNA, sediments, sequence analysis, sodium chloride, Arctic region, Chukchi Sea
An aerobic, Gram-negative, yellow-pigmented non-motile rod-shaped bacterium, designated Ch38ᵀ, was isolated from a sediment sample collected from the Chukchi Sea in the Arctic Ocean. Comparative 16S rRNA gene sequence analysis positioned strain Ch38ᵀ into the genus Winogradskyella as a distinct line adjacent to Winogradskyella multivorans KCTC 23891ᵀ, sharing the highest similarities of 97.5%, 97.2%, and 97.1% with Winogradskyella eximia KCTC 12219ᵀ, Winogradskyella damuponensis KCTC 23552ᵀ, and Winogradskyella multivorans KCTC 23891ᵀ, respectively. Strain Ch38ᵀ grew at 5–36 °C and in the presence of 1–6% (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C₁₆:₀ 3-OH, anteiso-C₁₅:₀ followed by iso-C₁₅:₀ and iso-C₁₆:₁ as the major fatty acids. Polar lipids consisted of phosphatidylethanolamine, three unknown aminolipids, an unknown lipid and an unknown phospholipid. The DNA C + C content was 31.7 mol%. Based on the phylogenetic analysis and distinctive phenotypic characteristics, strain Ch38ᵀ is concluded to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella profunda sp. nov. is proposed. The type strain of the species is strain Ch38ᵀ (= KMM 9725ᵀ = KACC 19710ᵀ).