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Flavobacterium dasani sp. nov., a psychrotolerant bacterium isolated from Arctic soil

Chaudhary, Dhiraj Kumar, Kim, Dockyu, Kim, Jaisoo
Archives of microbiology 2019 v.201 no.1 pp. 81-86
DNA, Flavobacterium, enzymes, fatty acids, frigid soils, new species, pH, phosphatidylethanolamines, psychrophilic bacteria, quinones, ribosomal RNA, sequence homology, sodium chloride, Arctic region
A novel yellow-colored, Gram-stain-negative, aerobic, non-motile, catalase- and oxidase-positive, and rod-shaped psychrotolerant bacterium, designated strain PLR-18-3ᵀ, was isolated from Arctic soil and was subjected to polyphasic taxonomic study. Cells were able to grow at 0–30 °C, pH 6.0–10.5, and 0–3.0% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, this Arctic strain belonged to the genus Flavobacterium, with the closest neighbor being Flavobacterium noncentrifugens R-HLS-17ᵀ (96.2% sequence similarity). The strain contained MK-6 as a sole respiratory quinone, phosphatidylethanolamine as the major polar lipid, and summed feature 3 (C₁₆:₁ω7c and/or C₁₆:₁ω6c), iso-C₁₅:₀, iso-C₁₅:₀ G, iso-C₁₇:₀ 3-OH, iso-C₁₅:₀ 3-OH, anteiso-C₁₅:₀, and summed feature 9 (iso-C₁₇:₁ω9c and/or C₁₆:₀10-methyl) as the predominant fatty acids. The DNA G + C content was 37.9 mol%. On the basis of polyphasic data, strain PLR-18-3ᵀ represents a novel species of the genus Flavobacterium, for which the name Flavobacterium dasani sp. nov. is proposed. The type strain is PLR-18-3ᵀ (=KEMB 9005-713ᵀ=KACC 19627ᵀ=NBRC 113347ᵀ).