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Paenibacillus amylolyticus 27C64 has a diverse set of carbohydrate-active enzymes and complete pectin deconstruction system

Keggi, Christian, Doran-Peterson, Joy
Journal of industrial microbiology & biotechnology 2019 v.46 no.1 pp. 1-11
Paenibacillus amylolyticus, cellulose, endo-1,4-beta-glucanase, enzyme activity, gene expression regulation, genes, methylation, pectins, polygalacturonase, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, xylan, xylanases
A draft genome of Paenibacillus amylolyticus 27C64 was assembled and a total of 314 putative CAZymes in 108 different families were identified. Comparison to well-studied polysaccharide-degrading organisms revealed that P. amylolyticus 27C64 has as many or more putative CAZymes than most of these organisms. Four different pectic substrates and xylan supported growth but cellulose was not utilized. Measurement of enzyme activities in culture supernatants revealed low levels of cellulase activity, high levels of xylanase activity, and pectinase activities that adapted to the specific polysaccharides provided. Relative expression levels of each putative pectinase in cells grown with and without three different pectic substrates were evaluated with RT-qPCR and distinct sets of genes upregulated in response to homogalacturonan, methylated homogalacturonan, and rhamnogalacturonan I were identified. It is also noted that this organism’s pectinolytic system differs from other well-studied systems and contains enzymes which are of value for further study.