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Molecular characterization of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar by rep-PCR analysis

Author:
Kyaw, Htet Wai Wai, Tsuchiya, Kenichi, Matsumoto, Masaru, Aye, Seint San, Iiyama, Kazuhiro, Kurose, Daisuke, Horita, Mitsuo, Furuya, Naruto
Source:
Journal of general plant pathology 2019 v.85 no.1 pp. 33-38
ISSN:
1345-2630
Subject:
DNA fingerprinting, Ralstonia solanacearum, bacterial wilt, cluster analysis, crops, genetic variation, host plants, phylotype, polymerase chain reaction, provenance, Myanmar
Abstract:
Seventy strains of Ralstonia solanacearum from solanaceous crops in Myanmar were molecularly characterized by rep-PCR DNA fingerprinting. Cluster analysis based on the fingerprints (total 33 fingerprint types) revealed seven main clusters at 80% similarity level. Strains in phylotype I showed 29 fingerprint types and were divided into six clusters, most of which were closely correlated with host plant, geographic origin, sequevar and/or biovar. In contrast, phylotype II strains showed four DNA fingerprint types within a single cluster. These results suggest that genetic diversity of the strains is endemic and that phylotype I and II may be invasive strains.
Agid:
6273002