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Study on the relationship between the concentration and type of fungal bio-aerosols at indoor and outdoor air in the Children’s Medical Center, Tehran, Iran

Sedighe Karimpour Roshan, Hatam Godini, Bahram Nikmanesh, Heidar Bakhshi, Arezoo Charsizadeh
Environmental monitoring and assessment 2019 v.191 no.2 pp. 48
Aspergillus, Cladosporium, Penicillium, adverse effects, aerosols, agar, air, bone marrow transplant, children, chloramphenicol, fungi, glucose, hospitals, mycelium, Iran
Fungal bio-aerosols are of concern due to their adverse health effects, especially in indoor environments. The aim of this study was to evaluate the relationship between the concentration and type of fungal bio-aerosols in the indoor and outdoor of Children’s Medical Center in Tehran, Iran. In the present descriptive-analytical study, the fungal bio-aerosols’ concentrations in both indoor and outdoor of the hospital air were measured. The measurements were carried out by the Anderson method using a Quick Take 30 pump at 28.3 L min⁻¹ and 2.5 min sampling that was placed on a Sabouraud dextrose agar with chloramphenicol. The average concentrations of total fungal bio-aerosols in the hospital indoor and outdoor air were 40.48 and 119.6 CFU/m³, respectively. Onco-hematology and bone marrow transplantation wards were the most and least contaminated units, respectively (11.09 CFU/m³ vs 1.47 CFU/m³). The most common fungi isolated from the indoor environment were Penicillium spp. (45.86%) which was followed by Cladosporium spp. (31.92%), Aspergillus section Nigri (6.26%), sterilized mycelia (5.05%), and Aspergillus section Flavi (2.83%). Cladosporium spp. (61.10 CFU/m³) and Penicillium spp. (18.56 CFU/m³) had the highest mean concentrations in outdoor and indoor air, respectively. The indoor-to-outdoor ratio of fungal aerosols was < 1 at most sampling sites, indicating that the indoor fungal bio-aerosols may have originated from the outdoor environment.