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Genetic analysis and mapping of a striped rind gene (st3) in melon (Cucumis melo L.)
- Liu, Liu, Sun, Tingting, Liu, Xiangyu, Guo, Yu, Huang, Xin, Gao, Peng, Wang, Xuezheng
- Euphytica 2019 v.215 no.2 pp. 20
- Cucumis melo, DNA, chromosome mapping, chromosomes, dominant genes, genetic analysis, high-throughput nucleotide sequencing, melons, plant breeding
- As an important commodity trait in melons, the rind is the most direct standard of evaluation for consumers. In this study, we obtained an F₂ melon population derived from self-crossing an F₁ generation from a cross between X010 (green rind with stripes) and M1-113 (white rind without stripes) and performed genetic analysis and mapping. Genetic analysis revealed that a single dominant gene (st3) controlled the striped rind trait in melons. According to bulked segregant analysis, two DNA bulks were constructed (20 striped and 20 solid-colored rind plants). Next-generation sequencing was used on two parental lines (X010 and M1-113) and two DNA bulks. The st3 gene was identified on chromosome 4 within 2.7 Mbp region. Fourty cleaved amplified polymorphic sequences (CAPS) markers were developed, and sixteen CAPS markers with polymorphisms on chromosome 4 were used for constructing a genetic linkage map, and identifying recombinants in a large F₂ population with six new CAPS markers. The location of the st3 gene was narrowed to 172.8 kbp on chromosome 4 between markers M-4-28 (825,401 bp) and M-4-27 (998,261 bp). This study provides a theoretical basis for the fine mapping, cloning and functional characterization of st3.