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Effects of Fe deficiency on the protein profile of Brassica napus phloem sap

Author:
Gutierrez-Carbonell, Elain, Lattanzio, Giuseppe, Albacete, Alfonso, Rios, Juan Jose, Kehr, Julia, Abadia, Anunciacion, Grusak, Michael A., Abadia, Javier, Lopez-Millan, Ana Flor
Source:
Proteomics 2015 v.15 no.22 pp. 3835-3853
Subject:
Brassica napus, calcium, flowering, homeostasis, hormones, iron, nutrient deficiencies, polyacrylamide gel electrophoresis, proteins, proteomics, sap, sieve elements, signal transduction
Abstract:
The aim of this work was to study the effect of Fe deficiency on the protein profile of phloem sap exudates from Brassica napus using 2-DE (IEF-SDS PAGE). The experiment was repeated thrice and two technical replicates per treatment were done. Two hundred sixty-three spots were consistently detected (found in all six replicates within each treatment) and 15.6% (41) of them showed significant changes in relative abundance (22 decreasing and 19 increasing) as a result of Fe deficiency. Among of them, 83% (34 spots), were unambiguously identified. Functional categories containing the largest number of protein species showing changes in relative abundance as a consequence of Fe deficiency were signaling and regulation (32%) and stress and redox homeostasis (17%). First, results indicate that Fe deficiency elicits major changes in signaling pathways involving Ca and hormones, which are generally associated with flowering and developmental processes. Second, Fe-deficiency causes an alteration in ROS homeostasis processes. Finally, Fe-deficiency induced decreases in the abundances of proteins involved in sieve element repair, suggesting that Fe-deficient plants may have an impaired capacity to heal sieve elements upon injury; this is also in line with the increased volume of phloem sap collected from Fe-deficient plants.
Handle:
10113/62744