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Effects of Fe deficiency on the protein profile of Brassica napus phloem sap

Elain Gutierrez-Carbonell, Giuseppe Lattanzio, Alfonso Albacete, Juan Jose Rios, Julia Kehr, Anunciacion Abadia, Michael A. Grusak, Javier Abadia, Ana Flor Lopez-Millan
Proteomics 2015 v.15 no.22 pp. 3835-3853
Brassica napus, calcium, flowering, homeostasis, hormones, iron, nutrient deficiencies, polyacrylamide gel electrophoresis, proteins, proteomics, sap, sieve elements, signal transduction
The aim of this work was to study the effect of Fe deficiency on the protein profile of phloem sap exudates from Brassica napus using 2-DE (IEF-SDS PAGE). The experiment was repeated thrice and two technical replicates per treatment were done. Two hundred sixty-three spots were consistently detected (found in all six replicates within each treatment) and 15.6% (41) of them showed significant changes in relative abundance (22 decreasing and 19 increasing) as a result of Fe deficiency. Among of them, 83% (34 spots), were unambiguously identified. Functional categories containing the largest number of protein species showing changes in relative abundance as a consequence of Fe deficiency were signaling and regulation (32%) and stress and redox homeostasis (17%). First, results indicate that Fe deficiency elicits major changes in signaling pathways involving Ca and hormones, which are generally associated with flowering and developmental processes. Second, Fe-deficiency causes an alteration in ROS homeostasis processes. Finally, Fe-deficiency induced decreases in the abundances of proteins involved in sieve element repair, suggesting that Fe-deficient plants may have an impaired capacity to heal sieve elements upon injury; this is also in line with the increased volume of phloem sap collected from Fe-deficient plants.