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Application of a proton quantitative nuclear magnetic resonance spectroscopy method for the determination of actinodaphnine in Illigera aromatica and Illigera henryi

Dong, Jian-Wei, Li, Xue-Jiao, Shi, Jun-You, Liu, Kai-Quan
Natural medicines 2019 v.73 no.1 pp. 312-317
Hernandiaceae, alkaloids, blood circulation, herbal medicines, herbs, molybdenum, nuclear magnetic resonance spectroscopy, quality control, tuberculosis
Illigera aromatica S. Z. Huang et S. L. Mo and Illigera henryi W. W. Sm., belonging to the genus Illigera (Hernandiaceae), are used as herbal medicines for promoting blood circulation and treating tuberculosis. Actinodaphnine, the major bioactive alkaloid, plays an important role in the quality controls of the herbs. In the present study, a rapid, simple, accurate, and precise proton quantitative nuclear magnetic resonance (¹H-qNMR) method was developed to determine the content of actinodaphnine in I. aromatica and I. henryi. DMSO-d₆ enabled satisfactory separation of the signals to be integrated in ¹H NMR spectrum. 1,4-Dinitrobenzene was selected as an internal standard. The limits of determination and quantitation were 0.005 and 0.038 mg/mL, respectively. This work implied that ¹H-qNMR represents a feasible alternative to HPLC-based methods for quantitation of actinodaphnine in I. aromatica and I. henryi and is suitable for the quality control of I. aromatica and I. henryi.