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Phycocyanin Production by Aphanothece microscopica Nägeli in Synthetic Medium Supplemented with Sugarcane Vinasse

Morais, Dayane V., Bastos, Reinaldo G.
Applied biochemistry and biotechnology 2019 v.187 no.1 pp. 129-139
Aphanothece, carbon, freezing, nitrogen content, phycocyanin, specific growth rate, sugarcane, thawing, vinasse
This study focused on the evaluation of mixotrophic and heterotrophic production of phycocyanin by A. microscopica, analysis of kinetic parameters, the effect of freezing and thawing on phycocyanin yield, and nutrient removal during heterotrophic growth. During mixotrophic growth, maximum phycocyanin yield (1.50 mgₚₕycₒcyₐₙᵢₙ g⁻¹bᵢₒₘₐₛₛ) was obtained after 12 h, while the heterotrophic cultivation yielded 1.39 mgₚₕycₒcyₐₙᵢₙ g⁻¹bᵢₒₘₐₛₛ. The mixotrophic cultivation of A. microscopica showed maximum specific growth rate of 0.025 h⁻¹, against 0.010 h⁻¹ for the photoautotrophic cultivation, and 0.08 h⁻¹ in heterotrophic conditions. The mixotrophic cultivation had a specific rate of phycocyanin production of 9.86 mgₚₕycₒcyₐₙᵢₙ mgbᵢₒₘₐₛₛ⁻¹ h⁻¹, while the photoautotrophic had 2.81 mgₚₕycₒcyₐₙᵢₙ mgbᵢₒₘₐₛₛ⁻¹ h⁻¹, and the heterotrophic, 49.18 mgₚₕycₒcyₐₙᵢₙ mgbᵢₒₘₐₛₛ⁻¹ h⁻¹. Carbon and nitrogen contents present in sugarcane vinasse were decreased in 16.69 and 15.97%, respectively, after 6 h of heterotrophic growth. Thus, it was shown that the mixotrophic production of phycocyanin by Aphanothece microscopica Nägeli in BG11 medium supplemented with vinasse is feasible.