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Analytical Method Validation for Determining Organophosphorus Pesticides in Baby Foods by a Modified Liquid–Liquid Microextraction Method and Gas Chromatography–Ion Trap/Mass Spectrometry Analysis

Notardonato, Ivan, Russo, Mario Vincenzo, Vitali, Matteo, Protano, Carmela, Avino, Pasquale
Food analytical methods 2019 v.12 no.1 pp. 41-50
food matrix, freeze drying, heptane, infant foods, infants, liquid-phase microextraction, mass spectrometry, organophosphorus pesticides, solvents, ultrasonics
Baby food is made specifically for babies between the ages of 4/6 months up to 2 years, and it comes in multiple varieties and tastes. This paper proposes a protocol for a rapid and reliable determination of 19 organophosphorus pesticides (OPs) by means of a method based on ultrasound-vortex-assisted liquid–liquid microextraction coupled with a gas chromatography–ion trap mass spectrometry (UVALLME–GC-IT/MS). The method development starts from the extraction solvent evaluation, i.e., n-heptane; the solution, held for 5 min on the vortex mixer and for 6 min in an ultrasonic bath to 100 W for favoring the solvent dispersion and the determinant extraction, is centrifuged at 4000 rpm for 30 min: 1 μL of the organic extract is injected into the GC-IT/MS. All the analytical parameters investigated are deeply discussed; LODs/LOQs (0.2–1.3 and 0.5–2.9 ng/g) and recoveries (81–109%) are compared with other papers dealing the determination of OPs in baby food matrix. The whole method has been applied to real commercial freeze-dried and soft baby food samples: the results do not show any significant contaminant values.