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Building a Fluorescent Aptasensor Based on Exonuclease-Assisted Target Recycling Strategy for One-Step Detection of T-2 Toxin
- He, Deyun, Wu, Zhengzong, Cui, Bo, Xu, Enbo, Jin, Zhengyu
- Food analytical methods 2019 v.12 no.2 pp. 625-632
- T-2 toxin, analytical methods, aptasensors, complementary DNA, detection limit, fluorescence, magnetism, nanoparticles, oligonucleotides
- In this work, a rapid and accurate assay was successfully developed for T-2 toxin detection based on exonuclease-catalyzed target recycling strategy. Upconversion nanoparticles (UCNPs) were conjugated with T-2 aptamer and used as signal probes, while magnetic nanoparticles (MNPs) were conjugated with the complementary DNA of T-2 aptamer (cDNA) and used as capture probes. The results reveled that good linear correlation (R² = 0.9988) was achieved for T-2 toxin detection over the concentration range of 0.1–100 ng/mL with a detection limit as low as 0.035 ng/mL (S/N = 3). In addition, the reliability of the proposed method was also applied to the determination of T-2 toxin contents in real food samples and the average recoveries ranged from 95.97 to 104.00%. The sensing platform developed in our study demonstrated great potential for simple and sensitive detection of T-2 toxin contents in food samples.