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Characterization of Pleurotus djamor neohaplonts recovered by production of protoplasts and chemical dedikaryotization

Hernández, Abraham Sánchez, Valenzuela Cobos, Juan Diego, Martínez, Joel Herrera, Arce, Ramón Villanueva, de las Mercedes Gómez y Gomez, Yolanda, Segura, Paola Berenice Zarate, Aguilar, María Eugenia Garín, Lara, Hermilo Leal, del Toro, Gustavo Valencia
3 Biotech 2019 v.9 no.1 pp. 24
Pleurotus djamor, hybrids, microsatellite repeats, phenotype, protoplasts
Production of hybrid strains is accomplished by mating monosporic isolates or neohaplonts, obtained either by chemical dedikaryotization or by production of protoplast. However, differences in growth rate among recovered neohaplonts have been reported. The presence of phenotypic and genetic changes among the neohaplonts recovered either by chemical dedikaryotization or by production of protoplast, was evaluated by measuring growth and morphology, and by molecular characterization using six ISSR markers to identify polymorphisms. Neohaplonts recovered by both methods presented variation in growth rate depending on their compatibility type and recovery method. Using ISSR markers, 59.2% polymorphism was established. Neohaplonts recovered by both monokaryotization procedures presented differences in growth rate and polymorphism.