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Annexin A8 (ANXA8) regulates proliferation of porcine endometrial cells via Akt signalling pathway

Jiang, Xiaona, Xue, Songyi, Kang, Tingting, Liu, Huijing, Ren, Huihui, Hua, Renwu, Ni, Debin, Lei, Minggang
Reproduction in domestic animals 2019 v.54 no.1 pp. 3-10
Western blotting, anticoagulants, blood coagulation, endometrium, epidermal growth factor, fluorescent antibody technique, gene expression, gene expression regulation, genes, interphase, leukemia, messenger RNA, mice, non-specific serine/threonine protein kinase, phosphorylation, pregnancy, proliferating cell nuclear antigen, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, signal transduction, swine
Annexin A8 (ANXA8) gene, a member of the annexin family, encodes an anticoagulant protein involved in blood coagulation cascade and acts as an indirect inhibitor of the thromboplastin‐specific complex. However, little is known about the function of ANXA8 in porcine endometrial cells so far. Here, ANXA8 mRNA was found to be abundant in porcine endometrium on days 11–13 of pregnancy. Real‐time RT–PCR analysis indicated that the mRNA expression of the leukaemia inhibitory factor (LIF) and the epidermal growth factor (EGF) was upregulated by ANXA8 in porcine endometrial cells. Immunofluorescence technology and cell cycle analysis revealed that ANXA8 promoted the proliferation of endometrial cells, as evidenced by the abundant proliferating cell nuclear antigen (PCNA) expression and an increase in the S phase. Western blot analysis results indicated that ANXA8 activated the phosphorylation of the target protein kinase B (Akt) protein. Immunofluorescence technology results showed that the PCNA protein had no significant change in porcine endometrial cells with both ANXA8 overexpression and the addition of Akt inhibitor. Furthermore, the number of implantation sites was significantly reduced by injection of mus‐siRNA‐ANXA8 into the uterine horn of mice. Collectively, these results suggest that ANXA8 promotes the proliferation of endometrial cells through the Akt signalling pathway.