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Overexpressing wheat low-molecular-weight glutenin subunits in rice (Oryza sativa L. japonica cv. Koami) seeds

Cho, Kyoungwon, Jo, Yeong-Min, Lim, Sun-Hyung, Kim, Joo Yeol, Han, Oksoo, Lee, Jong-Yeol
3 Biotech 2019 v.9 no.2 pp. 49
Oryza sativa, cultivars, dough, endosperm, extensibility, genes, glutenins, polyacrylamide gel electrophoresis, rice, seed storage proteins, seeds, transgenic plants, viscoelasticity, wheat, wheat gluten
Genes encoding wheat low-molecular-weight glutenin subunits (LMW-GSs) that confer dough strength and extensibility were previously identified from Korean wheat cultivars. To improve low viscoelasticity of rice (Oryza sativa L.) dough caused by the lack of seed storage proteins comparable to wheat gluten, two genes, LMW03 and LMW28, encoding LMW-GSs are cloned from Korean wheat cultivar Jokyoung. The LMW genes are inserted into binary vectors under the control of the rice endosperm-specific Glu-B1 promoter. Transgenic rice plants expressing LMW03 or LMW28 in their seeds are generated using Agrobacterium-mediated transformation. The expression of recombinant wheat LMW-GS in the transgenic rice seeds was confirmed by SDS-PAGE and immunoblot analysis. Their accumulation in the endosperm and aleurone layers of rice seeds was observed through in situ immuno-hybridization.