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Effect of nutrients on the growth of a new alpine strain of Haematococcus (Chlorophyceae) from New Zealand

Mazumdar, Nilanjana, Novis, Phil M., Visnovsky, Gabriel, Gostomski, Peter A.
Phycological research 2019 v.67 no.1 pp. 21-27
Haematococcus, aquaculture, astaxanthin, biomass production, biotin, carbon, cobalt, copper, culture flasks, industry, iron, manganese, microalgae, molybdenum, nitrogen, nutrients, sodium nitrate, thiamin, vitamin B12, zinc, New Zealand
The microalga Haematococcus lacustris is a source of astaxanthin used widely in aquaculture, pharmaceutical, and cosmetic industries. A new strain of Haematococcus (LCR‐26C‐1f) isolated from the New Zealand alpine zone was evaluated in this study. The influence of vitamins, micronutrients, various carbon and nitrogen sources were investigated to maximize biomass production in batch cultures using shake flasks. Supplementation of vitamins consisting of thiamine, biotin, and cyanocobalamin improved the cell density by 40% over the vitamin‐free medium. Out of the individual vitamins tested, thiamine was shown to be necessary to maintain high cell densities. The best nitrogen source tested was nitrate in the form of sodium nitrate, at a 40 mM concentration. Heterotrophic growth yielded much lower cell densities compared to autotrophic growth. The micronutrients iron and manganese were essential for growth. However, the best growth was obtained using a micronutrient mix that included iron, copper, cobalt, zinc, manganese and molybdenum.