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FANCD2 protects genome stability by recruiting RNA processing enzymes to resolve R‐loops during mild replication stress

Author:
Okamoto, Yusuke, Abe, Masako, Itaya, Akiko, Tomida, Junya, Ishiai, Masamichi, Takaori‐Kondo, Akifumi, Taoka, Masato, Isobe, Toshiaki, Takata, Minoru
Source:
TheFEBS journal 2019 v.286 no.1 pp. 139-150
ISSN:
1742-464X
Subject:
DNA, DNA-directed RNA polymerase, Fanconi anemia, chromatin, genes, hybrids, messenger RNA, proliferating cell nuclear antigen
Abstract:
R‐loops, which consist of DNA : RNA hybrids and displaced single‐strand DNA, are a major threat to genome stability. We have previously reported that a key Fanconi anemia protein, FANCD2, accumulates on large fragile genes during mild replication stress in a manner depending on R‐loops. In this study, we found that FANCD2 suppresses R‐loop levels. Furthermore, we identified FANCD2 interactions with RNA processing factors, including hnRNP U and DDX47. Our data suggest that FANCD2, which accumulates with R‐loops in chromatin, recruits these factors and thereby promotes efficient processing of long RNA transcripts. This may lead to a reduction in transcription–replication collisions, as detected by PLA between PCNA and RNA Polymerase II, and hence, lowered R‐loop levels. We propose that this mechanism might contribute to maintenance of genome stability during mild replication stress.
Agid:
6279494