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Mapping of the male sterile mutant gene ftms in Brassica rapa L. ssp. pekinensis via BSR-Seq combined with whole-genome resequencing

Tan, Chong, Liu, Zhiyong, Huang, Shengnan, Feng, Hui
Theoretical and applied genetics 2019 v.132 no.2 pp. 355-370
Arabidopsis, Brassica rapa, Chinese cabbage, breeding, centromeres, cobalt, exine, exons, galactosyltransferases, gamma radiation, genetic analysis, genotyping, hybrids, male fertility, male sterility, microspores, mutants, phenotype, pollen, quantitative polymerase chain reaction, radionuclides, single nucleotide polymorphism
KEY MESSAGE: A male sterile mutant was created by ⁶⁰Co γ-rays of microspores isolated from Chinese cabbage DH line ‘FT’. A candidate gene for the male sterile trait was identified as Bra010198. Male sterility is used for hybrid seed production in Chinese cabbage. In this study, we derived a male sterile mutant (ftms) from Chinese cabbage DH line ‘FT’ by irradiating microspores with ⁶⁰Co γ-rays and realized the rapid trait transformation from male fertility to sterility for creating valuable breeding materials. Genetic analysis indicated that the male sterile trait is controlled by a single recessive nuclear gene, ftms. Microspore development in mutant ftms was aborted at the tetrad stage and associated with severely retarded degeneration and vacuolation of tapetum. Using BSR-seq analysis, the candidate region for ftms was mapped on chromosome A05. A large F₂ population was created, and the region was narrowed to approximately 1.7-Mb between markers Indel20 and Indel14 via linkage analysis. The recombination frequency was extremely suppressed because the region was located on the chromosome A05 centromere. Whole-genome resequencing of mutant ftms and wild-type ‘FT’ aligned only one nonsynonymous SNP to Bra010198; this gene is a homolog of Arabidopsis KNS4/UPEX1, which encodes a putative β-(1,3)-galactosyltransferase that controls pollen exine development. Comparative sequencing verified the SNP position on the fifth exon of Bra010198 in mutant ftms. Further genotyping revealed that the male sterile phenotype was fully co-segregated with this SNP. Quantitative real-time PCR indicated that Bra0101918 specifically expressed in stamen. The data presented herein suggested that Bra010198 is a strong candidate gene for ftms. Hence, we developed a male sterile line for potential application in breeding and expanded the knowledge about the molecular mechanism underlying male sterility in Chinese cabbage.