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Phylloseptin-PBa1, -PBa2, -PBa3: Three novel antimicrobial peptides from the skin secretion of Burmeister's leaf frog (Phyllomedusa burmeisteri)

Author:
Wu, Youjia, Wang, Lei, Zhou, Mei, Chen, Tianbao, Shaw, Chris
Source:
Biochemical and biophysical research communications 2019 v.509 no.3 pp. 664-673
ISSN:
0006-291X
Subject:
Cruziohyla, Gram-positive bacteria, Phyllomedusa, antimicrobial peptides, antimicrobial properties, antineoplastic activity, bioactive compounds, cDNA libraries, complementary DNA, frogs, hemolysis, secretion, tandem mass spectrometry, yeasts
Abstract:
Skin secretions are known as a highly-complex mixture of abundant and diverse bioactive molecules and its study has attracted increasing attention over recent years. Phylloseptin is a unique family of antimicrobial peptides which have been only isolated from frogs of the Phyllomedusinae subfamily. Here, three novel peptide precursors were successfully cloned from a cDNA library, which was constructed from the skin secretion of Phyllomedusa burmeisteri, as pair of primers (one nested universal primer and a designed degenerate sense primer) were employed for “shotgun” cloning. The encoded mature peptides were validated by MS/MS sequencing, and subsequently termed as Phylloseptin-PBa1, -PBa2 and -PBa3. Phylloseptin-PBa1 and -PBa2 were demonstrated to possess potent antimicrobial activities against Gram-positive bacteria and yeast, as well as broad-spectrum anticancer activities, while they possess varying haemolytic activity at the effective concentration. In contrast, Phylloseptin-PBa3 was found to exhibit a strong haemolytic activity even though it was only found to possess a weak antimicrobial activity and inconspicuous anticancer activity.
Agid:
6280582