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MicroRNA-135a-5p promotes neuronal differentiation of pluripotent embryonal carcinoma cells by repressing Sox6/CD44 pathway

Author:
Li, Wang, Fang, Jie, Shen, Jingxuan, Liu, Xin, Hou, Jun, Zhu, Yingqi, Ma, Xinming
Source:
Biochemical and biophysical research communications 2019 v.509 no.2 pp. 603-610
ISSN:
0006-291X
Subject:
gene expression regulation, genes, microRNA, neoplasm cells, neurons
Abstract:
MicroRNA-135a-5p has been reported to play a potential role in the generation of new neurons. However, the underlying targets of miR-135a-5p in regulating neuronal differentiation have been poorly understood. Our study recently has uncovered that Sox6 and CD44 genes were significantly downregulated during neuronal differentiation of P19 cells, a multipotent cell type. We then found that Sox6 directly bound to the promoter of CD44. Importantly, we identified Sox6 as a direct target of miR-135a-5p. Additionally, we demonstrated that miR-135a-5p is crucial for the neuronal differentiation of P19 cells. More significantly, we found that Sox6 overexpression could overturn miR-135a-5p-mediated neuronal differentiation and dendrite development. In conclusion, these findings indicated that miR-135a-5p/Sox6/CD44 axis provides an important molecular target mechanism for neurodifferentiation.
Agid:
6280588