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Functional characterization of squalene epoxidase and NADPH-cytochrome P450 reductase in Dioscorea zingiberensis

Author:
Song, Wei, Yan, Shan, Li, Yi, Feng, Shan, Zhang, Jia-jiao, Li, Jia-ru
Source:
Biochemical and biophysical research communications 2019 v.509 no.3 pp. 822-827
ISSN:
0006-291X
Subject:
Dioscorea zingiberensis, NADP (coenzyme), amino acids, biosynthesis, catalytic activity, endoplasmic reticulum, epoxidation reactions, leaves, medicinal plants, methyl jasmonate, nucleotide sequences, plant tissues, recombinant proteins, squalene, squalene monooxygenase, steroid saponins, sterols, triterpenoids
Abstract:
Dioscorea zingiberensis is a perennial medicinal herb rich in a variety of pharmaceutical steroidal saponins. Squalene epoxidase (SE) is the key enzyme in the biosynthesis pathways of triterpenoids and sterols, and catalyzes the epoxidation of squalene in coordination with NADPH-cytochrome P450 reductase (CPR). In this study, we cloned DzSE and DzCPR gene sequences from D. zingiberensis leaves, encoding proteins with 514 and 692 amino acids, respectively. Recombinant proteins were successfully expressed in vitro, and enzymatic analysis indicated that, when SE and CPR were incubated with the substrates squalene and NADPH, 2,3-oxidosqualene was formed as the product. Subcellular localization revealed that both the DzSE and DzCPR proteins are localized to the endoplasmic reticulum. The changes in transcription of DzSE and DzCPR were similar in several tissues. DzSE expression was enhanced in a time-dependent manner after methyl jasmonate (MeJA) treatments, while DzCPR expression was not inducible.
Agid:
6280622