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Recruitment of the protein phosphatase-1 catalytic subunit to promoters by the dual-function transcription factor RFX1

Author:
Lubelsky, Yoav, Shaul, Yosef
Source:
Biochemical and biophysical research communications 2019 v.509 no.4 pp. 1015-1020
ISSN:
0006-291X
Subject:
DNA-binding proteins, DNA-directed RNA polymerase, amino acid sequences, genome, phosphoprotein phosphatase, protein subunits, transcription factors
Abstract:
RFX proteins are a family of conserved DNA binding proteins involved in various, essential cellular and developmental processes. RFX1 is a ubiquitously expressed, dual-activity transcription factor capable of both activation and repression of target genes.The exact mechanism by which RFX1 regulates its target is not known yet. In this work, we show that the C-terminal repression domain of RFX1 interacts with the Serine/Threonine protein phosphatase PP1c, and that interaction with RFX1 can target PP1c to specific sites in the genome. Given that PP1c was shown to de-phosphorylate several transcription factors, as well as the regulatory C-terminal domain of RNA Polymerase II the recruitment of PP1c to promoters may be a mechanism by which RFX1 regulates the target genes.
Agid:
6280623