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Chemo-enzymatic synthesis of p-nitrophenyl β-D-galactofuranosyl disaccharides from Aspergillus sp. fungal-type galactomannan
- Ota, Ryo, Okamoto, Yumi, Vavricka, Christopher J., Oka, Takuji, Matsunaga, Emiko, Takegawa, Kaoru, Kiyota, Hiromasa, Izumi, Minoru
- Carbohydrate research 2019 v.473 pp. 99-103
- Aspergillus, Streptomyces, cell walls, disaccharides, enzymatic hydrolysis, fungi, galactomannans, galactosidases, glycoconjugates, transferases
- β-d-Galactofuranose (Galf) is a component of polysaccharides and glycoconjugates. There are few reports about the involvement of galactofuranosyltransferases and galactofuranosidases (Galf-ases) in the synthesis and degradation of galactofuranose-containing glycans. The cell walls of filamentous fungi in the genus Aspergillus include galactofuranose-containing polysaccharides and glycoconjugates, such as O-glycans, N-glycans, and fungal-type galactomannan, which are important for cell wall integrity. In this study, we investigated the synthesis of p-nitrophenyl β-d-galactofuranoside and its disaccharides by chemo-enzymatic methods including use of galactosidase. The key step was selective removal of the concomitant pyranoside by enzymatic hydrolysis to purify p-nitrophenyl β-d-galactofuranoside, a promising substrate for β-d-galactofuranosidase from Streptomyces species.