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Characterization of the ScghC1q-1 gene in Sinonovacula constricta and its role in innate immune responses
- Li, Yan, Niu, Donghong, Bai, Yuqi, Lan, Tianyi, Peng, Maoxiao, Dong, Zhiguo, Li, Jiale
- Developmental and comparative immunology 2019 v.94 pp. 16-21
- Gram-negative bacteria, Staphylococcus aureus, Veneroida, Vibrio anguillarum, amino acids, complement, gene expression regulation, genes, hemolymph, immune response, innate immunity, messenger RNA, open reading frames, tissues
- C1q is an important immune gene that can mediate a variety of immune regulatory functions, and is involved in complement pathway activation. In the present study, a ghC1q gene from the razor clam Sinonovacula constricta was identified and named ScghC1q-1. The complete ScghC1q-1 gene is 692 bp in length, with an open reading frame (ORF) of 489 bp encoding a protein of 162 amino acids. ScghC1q-1 mRNA was widely expressed in various tissues, and transcript levels in the hemolymph were significantly up-regulated following Staphylococcus aureus or Vibrio anguillarum challenge. Recombinant ScghC1q-1 protein was found to agglutinate both Gram-positive and Gram-negative bacteria. These results indicate that ScghC1q-1 plays an essential role in the immune defense of S. constricta.