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An AP2/ERF gene, IbRAP2-12, from sweetpotato is involved in salt and drought tolerance in transgenic Arabidopsis

Li, Yan, Zhang, Huan, Zhang, Qian, Liu, Qingchang, Zhai, Hong, Zhao, Ning, He, Shaozhen
Plant science 2019 v.281 pp. 19-30
Arabidopsis, abiotic stress, abscisic acid, biosynthesis, drought tolerance, ethephon, gene overexpression, genes, heterologous gene expression, hydrogen peroxide, jasmonic acid, methyl jasmonate, polyethylene glycol, proline, salt tolerance, sodium chloride, sweet potatoes, tobacco, transcriptional activation, transcriptomics, transgenic plants, yeasts
The manipulation of APETALA2/ethylene responsive factor (AP2/ERF) genes in plants makes great contributions on resistance to abiotic stresses. Here, we cloned an AP2/ERF gene from the salt-tolerant sweetpotato line ND98 and named IbRAP2-12. IbRAP2-12 protein expressed in nuclear revealed by transient expression in tobacco epidermal cells, and IbRAP2-12 exhibited transcriptional activation using heterologous expression assays in yeast. IbRAP2-12 was induced by NaCl (200 mM), 20% polyethylene glycol (PEG) 6000, 100 μM abscisic acid (ABA), 100 μM ethephon and 100 μM methyl jasmonate (MeJA). IbRAP2-12-overexpressing Arabidopsis lines were more tolerant to salt and drought stresses than wild type plants. Transcriptome analysis showed that genes involved in the ABA signalling, JA signalling, proline biosynthesis and reactive oxygen species (ROS) scavenging processes were up-regulated in IbRAP2-12 overexpression lines under salt and drought stresses. In comparing with WT, the contents of ABA, JA and proline were significantly increased, while hydrogen peroxide (H2O2) and the rate of water loss were significantly reduced in transgenic lines under salt and drought stresses. All these results demonstrated the roles of IbRAP2-12 in enhancing salt and drought tolerance in transgenic Arabidopsis lines. Thus, this IbRAP2-12 gene can be used to increase the tolerance ability during abiotic stresses in plants.