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Ethanol production from sugarcane bagasse: Use of different fermentation strategies to enhance an environmental-friendly process

de Araujo Guilherme, Alexandre, Dantas, Paulo Victor Fortunato, Padilha, Carlos Eduardo de Araújo, dos Santos, Everaldo Silvino, de Macedo, Gorete Ribeiro
Journal of environmental management 2019 v.234 pp. 44-51
Kluyveromyces marxianus, Saccharomyces cerevisiae, batch systems, beta-glucosidase, biofuels, biomass, bioreactors, cellulose, ethanol, ethanol production, fermentation, hydrogen peroxide, inoculum, lignocellulose, oils, saccharification, sugarcane bagasse, yeasts
Ethanol production by simultaneous saccharification and fermentation (SSF) using sugarcane bagasse as substrate was developed using batch and fed-batch mode. Acid, alkali, hydrothermal and hydrogen peroxide pretreatments to the sugarcane bagasse were tested. Experiments were carried out to optimize the enzyme load of cellulases and β-glucosidase. Four strains, two of Saccharomyces cerevisiae and two of Kluyveromyces marxianus yeast species were evaluate using SSF to produce ethanol. A kinetic study in bioreactor was carried out to optimize the SSF. The batch process was optimized using 1.0 g/L of inoculum, 15.0 FPU/g cellulose of cellulases and 6.0% of initial cellulose reaching 92.0% of theoretical ethanol yield after 18 h using the bagasse pretreate by acid-alkali and S. cerevisiae PE-2. The fed-batch process with enzyme load three times lower than that was used in batch process, obtained 88% of theoretical ethanol yield in 40 h. Therefore, the use of the lignocellulosic biomass (sugarcane bagasse) for producing a biofuel (ethanol) reduces the need for oil and is an environmental-friendly process.