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Kinetic and microbial response of activated sludge community to acute and chronic exposure to tetracycline

Author:
Pala-Ozkok, Ilke, Ubay-Cokgor, Emine, Jonas, Daniel, Orhon, Derin
Source:
Journal of hazardous materials 2019 v.367 pp. 418-426
ISSN:
0304-3894
Subject:
Arthrobacter, activated sludge, antibiotic resistance genes, bacteria, bacterial communities, biomass, chronic exposure, community structure, energy, growth models, high-throughput nucleotide sequencing, organic carbon, oxygen, oxygen consumption, tetracycline, wastewater
Abstract:
Current study aimed to discover both kinetic and microbial response of activated sludge biomass to continuous exposure to tetracycline, one of the most frequently detected antibiotics in wastewaters. Respirometric analysis and model evaluation of the oxygen utilization rate profiles generated at critical phases of the experimental period showed that, continuous exposure to tetracycline caused complete suppression of substrate storage aside from mild inhibition on the growth kinetics and it exerted a significant binding action with available organic carbon, leading to less oxygen consumption. Additionally, increase in endogenous decay rates by 1.5 fold was associated with maintenance energy dictated by the presence and production of antibiotic resistance genes, as demonstrated by resistance gene profile. High-throughput sequencing results showed that continuously exposure to tetracycline caused a significant shift in the community structure at species level so that tetracycline resistant bacteria like Arthrobacter sp and Diaphorobacter sp dominated the bacterial community.
Agid:
6284347