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A novel onsite and visual molecular technique to authenticate saffron (Crocus sativus) and its adulterants based on recombinase polymerase amplification

Author:
Zhao, Mingming, Wang, Bo, Xiang, Li, Xiong, Chao, Shi, Yuhua, Wu, Lan, Meng, Xiangxiao, Dong, Gangqiang, Xie, Yiqiang, Sun, Wei
Source:
Food control 2019 v.100 pp. 117-121
ISSN:
0956-7135
Subject:
Crocus sativus, DNA, adulterants, adulterated products, cross reaction, herbs, immunoassays, prices, saffron, China
Abstract:
Saffron (Crocus sativus), one of the most expensive herbs in China, has been subject to various types of adulteration as a consequence of its high price and limited production. The present work describes a sensitive, efficient and simple technique for on-site saffron identification. Recombinase polymerase amplification (RPA) in combination with a universal lateral flow immunoassay strip (saffron RPA-LFD) was employed for rapid visual detection of saffron. This assay was able to detect saffron in less than 15 min at 37 °C. This assay was highly sensitive with detection limits of 1 pg genomic DNA, and highly specific, with no cross-reaction with common saffron adulterants. This proposed simple and sensitive assay is well-suited for rapid molecular detection of saffron, with results in the form of the presence (positive bands) or absence (no positive bands) of bands.
Agid:
6285944