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Glycosylation of human plasma lipoproteins reveals a high level of diversity, which directly impacts their functional properties

Author:
Sukhorukov, Vasily, Gudelj, Ivan, Pučić-Baković, Maja, Zakiev, Emile, Orekhov, Alexander, Kontush, Anatol, Lauc, Gordan
Source:
Biochimica et biophysica acta 2019 v.1864 no.5 pp. 643-653
ISSN:
1388-1981
Subject:
cholesterol, cholesteryl esters, chromatography, functional properties, glycosylation, high density lipoprotein, humans, low density lipoprotein, matrix-assisted laser desorption-ionization mass spectrometry, sialic acid, sialidase
Abstract:
Human plasma lipoproteins are known to contain various glycan structures whose composition and functional importance are starting to be recognized. We assessed N-glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism.N-glycomic profiles of native and neuraminidase-treated HDL and LDL were obtained using HILIC-UHPLC-FLD. Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N-glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells.HILIC-UHPLC-FLD analysis of HDL and LDL N-glycans released by PNGase F resulted in 22 and 18 distinct chromatographic peaks, respectively. The majority of N-glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues. The most abundant N-glycan structure in both HDL and LDL was a complex type biantennary N-glycan with one sialic acid (A2G2S1). Relative abundances of several N-glycan structures were dramatically altered by the neuraminidase treatment, which selectively removed sialic acid residues. Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p < 0.05). Cellular accumulation of cholesteryl esters in THP-1 cells was significantly higher after incubations with desialylated LDL particles as compared to native LDL (p < 0.05).N-glycome of human plasma lipoproteins reveals a high level of diversity, which directly impacts functional properties of the lipoproteins.
Agid:
6286649