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Bigbelly seahorse (Hippocampus abdominalis)-derived peptides enhance skeletal muscle differentiation and endurance performance via activated P38MAPK/AKT signalling pathway: An in vitro and in vivo analysis

Muthuramalingam, Karthika, Kim, Seo-Young, Kim, Youngmee, Kim, Hyun-Soo, Jeon, You-Jin, Cho, Moonjae
Journal of functional foods 2019 v.52 pp. 147-155
Danio rerio, Hippocampus abdominalis, aquatic organisms, capacitance, cell viability, fatigue strength, mice, models, muscle development, muscle fibers, myoblasts, peptides, regulatory proteins, signal transduction, skeletal muscle, swimming, therapeutics, water flow, South Korea
Big belly seahorse (Hippocampus abdominalis) is a well-known marine organism recognized for its pharmacological value. In this study, peptides derived from natively (Jeju-do, Republic of Korea) farmed seahorses were investigated for their potency on myoblast differentiation and endurance capacitance. Cell viability studies on C2C12 mouse myoblast cells showed that more than 80% of the cells treated with peptides were metabolically active. Morphological observation illustrated the loss of characteristic radial branching towards elongated and well-developed muscle fibers in the peptide-treated C2C12 cells. Immunoblot study on the in vitro translational expression level of key myogenic regulatory proteins (MyoD, MyoG, MyHC) and an in vivo endurance study (using zebrafish as a working model) demonstrated the significance of peptides on the myogenesis process and endurance swimming performance against water flow and gradient, respectively. Collectively, our findings suggest that seahorse-derived peptides can be used as a therapeutic nutrient supplement for improved endurance.