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Oosporein, an abundant metabolite in Beauveria caledonica, with a feedback induction mechanism and a role in insect virulence

Author:
Mc Namara, Louise, Dolan, Stephen K., Walsh, John M.D., Stephens, John C., Glare, Travis R., Kavanagh, Kevin, Griffin, Christine T.
Source:
Fungal biology 2019 v.123 no.8 pp. 601-610
ISSN:
1878-6146
Subject:
Beauveria bassiana, Galleria mellonella, Hylobius abietis, adults, antifeedants, biological control agents, biosynthesis, enzymes, forestry, hemocytes, immune system, immunomodulation, insect larvae, insects, metabolites, pests, proteomics, virulence
Abstract:
Oosporein was first identified from the insect pathogen Beauveria bassiana >50 y ago. Here, we investigate the insecticidal, anti-feedant and immunomodulation effects of oosporein produced by Beauveria caledonica on the forestry pest Hylobius abietis and model insect Galleria mellonella. We report a novel feedback induction mechanism regulating oosporein production in B. caledonica; exogenous oosporein induces the expression of the oosporein cluster, leading to increased abundance of oosporein biosynthetic enzymes, as shown by label-free quantitative proteomics. Oosporein did not have an anti-feedant effect on H. abietis adults – on the contrary, insects exposed to oosporein-treated food fed more than those exposed to untreated food only. Injected oosporein did not kill insect larvae but increased susceptibility of H. abietis to a subsequent infection. Oosporein did not act as a contact toxin on H. abietis adults and G. mellonella larvae at the concentrations tested. Therefore, it appears that oosporein promotes infection rather than directly killing insects; this could be mediated both by a reduction in haemocyte numbers and by alterations to the humoral immune system. This work makes a case for future research into the potential use of B. caledonica as a biocontrol agent through combinations with oosporein or with enhanced production of oosporein.
Agid:
6287430