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Enzymatically hydrolysed sago bagasse improves physiological, biochemical and molecular attributes of Solanum lycopersicum

Author:
Kumar, Sathiya, Chinnannan, Karthik, Thamilarasan, Senthil Kumar, Seralathan, Muhilvannan, Shanmuganathan, Rajasree, Padikasan, Indra Arulselvi
Source:
Biocatalysis and agricultural biotechnology 2019 v.17 pp. 499-506
ISSN:
1878-8181
Subject:
Fourier transform infrared spectroscopy, Solanum lycopersicum, X-ray diffraction, agricultural productivity, bagasse, biofertilizers, carbon, citrate (si)-synthase, enzymatic hydrolysis, gene expression regulation, genes, greenhouse experimentation, hydrolysates, hydrolysis, industry, isocitrate dehydrogenase, malate dehydrogenase, nitrate reductase, nitrogen, particle size, plant growth, quantitative polymerase chain reaction, sago, scanning electron microscopy, seed germination, sugars
Abstract:
The present study was aimed to investigate the plant biostimulant properties of sago bagasse (SB), an abundant by-product of sago industry. Sago bagasse hydrolysate (SBH) was produced by enzymatic hydrolysis and characterized by X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), particle size analyzer and Scanning electron microscopy (SEM) analysis. Further, plant growth promoting ability of the SBH was confirmed by seed germination and greenhouse experiments. In seed germination experiment, SBH treatment registered improved seed germination traits and also accelerated protein (3.13%) and sugar (9.53%) content as compared to control. Moreover, SBH treatment significantly increase expression of carbon assimilating enzymes like malate dehydrogenase (5.0%), citrate synthase (11.47%) isocitrate dehydrogenase (8.08%). Similarly, nitrogen assimilating enzymes such as nitrate reductase (15.14%) and glutamate synthase (10.98%) were also higher in SBH the treated plants. In addition, qPCR analysis demonstrated the efficiency of SBH by up-regulating carbon and nitrogen assimilating genes responsible for plant growth. Thus, the present finding strongly suggest that SBH with plant growth promoting properties could be utilized for the agricultural productivity as a low-cost ecofriendly biofertilizer.
Agid:
6287464