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Negative hydrostatic pressure is an unnoticed but significant source of contamination in tissue culture

Author:
Askari, N., De Klerk, G. J.
Source:
Acta horticulturae 2018 no.1212 pp. 85-88
ISSN:
0567-7572
Subject:
air, bacteria, hydrostatic pressure, liquids, tissue culture, transpiration, xylem vessels
Abstract:
Plants are characterized by a negative hydrostatic pressure, brought about by transpiration and by capillary activity of xylem vessels (Taiz and Zeiger, 2010). Because of this, a stem that is being cut sucks up what is nearby. Often this is air but it may also be liquid. The diameter of the xylem vessels is 50-100 μm, so when the liquid contains bacteria (that are typically 0.5-5.0 μm), they will enter deeply into the tissue (Askari et al., 2014; De Klerk et al., 2014). To our knowledge, this alleged source of contamination has never been examined.
Agid:
6287698