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Micropropagation of ornamental aquatic plants, Glossostigma, Microcarpaea and Limnophila 2. Effect of CaCl2・2H2O, KH2PO4, Fe-EDTA concentrations on the growth of explants©

Minamiyama, M., Noguchi, A., Amaki, W.
Acta horticulturae 2018 no.1212 pp. 387-390
Limnophila, aquatic plants, color, culture media, leaves, micropropagation, potassium dihydrogen phosphate, tissue culture
For each concentration of CaCl(2)・2H(2)O (1.5, 0.6 and 0.3 mM), KH2PO4 (0.63, 0.25, and 0.13 mM), and Fe-EDTA (50, 25, and 13 μM) in the tissue culture medium, the effects on the in vitro growth of three aquatic plants, Limnophila sp. (unidentified), Glossostigma elatinoides (Benth.) Hook.f., and Microcarpaea minima (K.D. Koenig ex Retz.) Merrill., were examined. On the result of CaCl2・2H(2)O, Limnophila and M. minima showed the highest value of plant fresh weight (FW) on medium supplemented at 0.3 mM. However, leaf yellowing and abnormal growth occurred at 0.3 mM in Limnophila. On the other hand, leaf color of M. minima became darker at the lower concentration. In G. elatinoides, the highest value of FW was obtained when the concentration was 0.6 mM. In all three species, lowering the concentration of KH(2)PO(4) decreased the FW of plants. There was a clear tendency for FW to increase with decreasing Fe-EDTA concentration in Limnophila and M. minima. On the other hand, FW was maximized at 25 µM of Fe-EDTA and when the concentration was lowered to 13 µM, FW remarkably decreased in G. elatinoides.