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Novel Strategy to Decipher the Regulatory Mechanism of 1-Naphthaleneacetic Acid in Strawberry Maturation

An, Li, Ma, Jingwei, Qin, Dongmei, Wang, Hong, Yuan, Yongliang, Li, Honglian, Na, Risong, Wu, Xujin
Journal of agricultural and food chemistry 2019 v.67 no.4 pp. 1292-1301
biochemical pathways, citric acid, genes, matrix-assisted laser desorption-ionization mass spectrometry, metabolites, metabolomics, naphthaleneacetic acid, nuclear magnetic resonance spectroscopy, ripening, strawberries, transcriptomics
1-Naphthaleneacetic acid (NAA) has long been used to regulate strawberry growth. However, its regulatory mechanisms are unclear. Here, a nuclear magnetic resonance (NMR)-based metabolomics approach was utilized to capture differential metabolites, then matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and transcriptomics as assisted methods to validate the significant findings of metabolomics. The metabolomics results suggested that NAA regulated strawberry growth via multiple metabolic pathways, and different NAA application times also influenced these regulatory effects. We also found an interesting phenomenon that citric acid had completely opposite changes when NAA was sprayed at two different ripening stages of the strawberries. Furthermore, MALDI-TOF MS validated the changes of citric acid and transcriptomics identified the related genes. The study demonstrated that the novel strategy of “metabolomics capture-MALDI-TOF MS and transcriptomics assisted validation” could offer a fresh insight for understanding the mechanism of the plant growth regulator in strawberry maturation.