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n-Butanol Extract of Lotus Seeds Exerts Antiobesity Effects in 3T3-L1 Preadipocytes and High-Fat Diet-Fed Mice via Activating Adenosine Monophosphate-Activated Protein Kinase

Wang, Zhenyu, Hu, Jiamiao, Hamzah, Siti Sarah, Ge, Shenghan, Lin, Yilin, Zheng, Baodong, Zeng, Shaoxiao, Lin, Shaoling
Journal of agricultural and food chemistry 2019 v.67 no.4 pp. 1092-1103
AMP-activated protein kinase, Lotus, adiponectin, blood lipids, butanol, cytotoxicity, gene expression regulation, genes, high fat diet, lipid composition, lipogenesis, lipolysis, mice, peroxisome proliferator-activated receptor alpha, peroxisome proliferator-activated receptor gamma, seeds, signal transduction, weight loss
In this study, the antiobesity effects of n-butanol extract of lotus seeds (LBE) were evaluated in cultured 3T3-L1 preadipocytes and in high-fat diet (HFD)-fed mice. LBE decreased lipid contents in mature 3T3-L1 cells without obvious cytotoxicity. Meanwhile, LBE supplementation also led to weight loss and improved plasma lipid profiles in HFD-fed mice. Furthermore, LBE could activate AMP-activated protein kinase (AMPK) accompanied by down-regulation of lipogenesis related genes (PPARγ, aP2, LPL, C/EBPα, FAS, SREBP-1c) and up-regulation of lipolysis genes (adiponectin and PPARα) in vitro and in vivo. Collectively, our data demonstrated LBE possesses antiadipogenic and antilipogenic activities which are, at least partially, mediated by the activation of AMPK signaling pathways.