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A novel NIR-emissive probe with large Stokes shift for hypochlorite detection and imaging in living cells

Author:
Liu, Jingwei, Yin, Zheng
Source:
Talanta 2019 v.196 pp. 352-356
ISSN:
0039-9140
Subject:
fluorescence emission spectroscopy, image analysis, moieties, ultraviolet-visible spectroscopy
Abstract:
Due to the importance of hypochlorite (ClO-) in the process of life, a near-infrared region (NIR) emissive probe (DCPO-DMTC) with large Stokes shift was synthesized for the selective detection of hypochlorite. This probe detects exogenous and endogenous hypochlorite via “oxidative deprotection” of dimethylthiocarbamate-protected phenolic hydroxyl groups. The response was monitored by time-course UV–Vis and fluorescent spectroscopy. The emission response of the probe to ClO− presented a good linear relationship in the 0–100 µM concentration range, and the LOD of this probe was 164 nM. The probe was used to successfully visualize endogenous ClO- generation in RAW 246.7 cells under external stimulation.
Agid:
6289667