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A rhodamine-deoxylactam based fluorescent probe for fast and selective detection of nitric oxide in living cells

Jiang, Wen-Li, Li, Yongfei, Liu, Hong-Wen, Zhou, Dong-Ye, Ou-Yang, Juan, Yi, Lanhua, Li, Chun-Yan
Talanta 2019 v.197 pp. 436-443
ascorbic acid, cysteine, dehydroascorbic acid, fluorescence, fluorescent dyes, nitric oxide, rhodamines
Nitric oxide (NO) plays vital roles in many physiological process and is closely related to many diseases. So far, a number of fluorescent probes have been constructed for the detection of NO successfully. However, the probes still suffer from long-time reaction and limited selectivity. Herein, a fluorescent probe named dRB-OPD is synthesized and used to recognize NO. The probe contains a deoxy-rhodamine B as fluorophore and o-phenylenediamino as reaction site. dRB-OPD shows fast response to NO within 40 s with 170-fold fluorescence enhancement. Moreover, the probe shows high selectivity towards NO over dehydroascorbic acid (DHA), ascorbic acid (AA), and methylglyoxal (MGO). Particularly, the probe can avoid the serious interference from cysteine (Cys) found in the rhodamine lactam-based fluorescent NO probes (RB-OPD). In addition, the probe is applied for the detection of exogenous and endogenous NO in the HepG2 and RAW 264.7 cells with satisfactory results.