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Construction of a novel far-red fluorescence light-up probe for visualizing intracellular peroxynitrite

Author:
Qu, Wangbo, Niu, Changhe, Zhang, Xiaoyu, Chen, Wei, Yu, Fabiao, Liu, Heng, Zhang, Xiuhua, Wang, Shengfu
Source:
Talanta 2019 v.197 pp. 431-435
ISSN:
0039-9140
Subject:
cell lines, fluorescence, fluorescent dyes, macrophages, moieties, neoplasms, neurodegenerative diseases, reactive oxygen species
Abstract:
Peroxynitrite (ONOO-) is an important reactive oxygen species (ROS), which can react with a variety of biologically active species and cause many diseases, such as cancer, neurodegenerative disorders. Herein, we develop a novel far-red fluorescent probe DCM-KA, which is equipping with α-ketoamide moiety caged DCM-NH2. The probe exhibits fluorescence off-on response to ONOO- over other biological interfering analytes by ONOO--induced α-ketoamide deprotection reaction. More importantly, the probe is biocompatible and has been successfully utilized to visualize endogenous ONOO- production in macrophage cell line J774A.1.
Agid:
6289747