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Magnetic nanospheres for convenient and efficient capture and release of hepatitis B virus DNA

Author:
Wen, Cong-Ying, Liu, Ting-Ting, Wu, Ling-Ling, Li, Yi-Min, Sun, Jia-Yi, Zeng, Jing-Bin
Source:
Talanta 2019 v.197 pp. 605-611
ISSN:
0039-9140
Subject:
DNA, Hepatitis B virus, blood serum, isolation techniques, magnetic separation, magnetism, melting, models, molecular biology, nanospheres, nucleotide sequences, operating costs, temperature
Abstract:
Nucleic acid isolation and purification are essential steps in molecular biology. Currently-used isolation methods focus on the extraction of all the nucleic acids from crude samples, yet ignore the specific nucleic acids of interest, which may induce the loss of the specific nucleic acids and hinder their analyses. Herein, a magnetic nanospheres (MNs)-based strategy for efficient capture and release of specific nucleic acids is developed. The DNA sequence of hepatitis B virus (HBV) is taken as a model to validate this method. The MNs are modified with the complementary strand of HBV DNA for specific capture based on hybridization reaction. Then, by melting at high temperature, the captured DNAs are detached from the MNs to achieve release. The capture and release process are performed conveniently with magnetic separation. High capture efficiency (over 80%) and nearly 100% release efficiency for HBV DNA are achieved respectively via 40 min and 5 min interaction. While non-target DNAs are hardly captured, indicative of good selectivity. Moreover, after releasing DNAs, the MNs are directly regenerated and can be reused without degrading performance, which greatly reduces the operation costs. Finally, this method is applied to serum samples without any pretreatment, which exhibits similar capture and release capacity with those in the ideal samples, indicating its great application potential in practice.
Agid:
6289769