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Photoinhibition of photosystem I under fluctuating light is linked to the insufficient ΔpH upon a sudden transition from low to high light

Author:
Huang, Wei, Yang, Ying-Jie, Zhang, Shi-Bao
Source:
Environmental and experimental botany 2019 v.160 pp. 112-119
ISSN:
0098-8472
Subject:
Arabidopsis thaliana, Bletilla striata, chlorophyll, light intensity, lighting, oxygen, photoinhibition, photosystem I, thylakoids
Abstract:
Despite photosystem I (PSI) is susceptible to photoinhibition under fluctuating light in wild-type angiosperms, the underlying mechanism is not well known. Because proton gradient (ΔpH) across the thylakoid membranes plays a crucial role in protecting PSI, we hypothesized that PSI photoinhibition under fluctuating light may be linked to the formation of ΔpH. To test this hypothesis, we measured chlorophyll fluorescence, PSI redox state, and the electrochromic shift signal during transition from low to high light in two angiosperms Bletilla striata (Orchidaceae) and Arabidopsis thaliana. The measurement of fast P700 redox kinetics indicated that the fast re-oxidation of P700 mediated by photo-reduction of O2 was absent in B. striata. Furthermore, the redox state of PSI is highly determined by ΔpH in both species. For the first 20 s after transition from low to high light, both species could not build up a sufficient ΔpH, which was accompanied by the over-reduction of P700. During prolonged illumination at high light, the sufficient ΔpH made PSI to be highly oxidized. These results demonstrated that PSI photoinhibition under fluctuating light in wild-type angiosperms occurred mainly at the first 20 s after an increase in light intensity, which was caused by the insufficient ΔpH.
Agid:
6290463