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Engineering retinal pigment epithelial cells regeneration for transplantation in regenerative medicine using PEG/Gellan gum hydrogels

Kim, Han Sol, Kim, David, Jeong, Yong Woon, Choi, Min Joung, Lee, Gi Won, Thangavelu, Muthukumar, Song, Jeong Eun, Khang, Gilson
International journal of biological macromolecules 2019 v.130 pp. 220-228
biocompatibility, cell adhesion, cell growth, cell transplantation, epithelial cells, epithelium, gellan gum, gene expression, hydrogels, medicine, polyethylene glycol, retina, reverse transcriptase polymerase chain reaction, tissue engineering
Retinal pigment epithelium (RPE) plays an important role in maintaining normal function and visual function of the retina, and the degeneration of RPE causes various retinal degenerative diseases. Currently, there is a lack of effective treatment for this, and it is being studied to produce a suitable scaffold for cell transplantation. In this experiment, Polyethylene glycol (PEG)/Gellan Gum (GG) hydrogel was prepared by adding harmless PEG and gellan gum, which is a biocompatible, degradable and widely used in modern tissue engineering. PEG/GG hydrogel was prepared with 0, 1, 3, 5 wt% PEG/GG according to the concentration of PEG, and ARPE-19 cells were used to confirm the cell attachment environment. As a result, it showed superior biocompatibility (>90%), cell adhesion and improved cell growth compared to gellan gum hydrogel. In addition, RT-PCR was used to confirm RPE-specific gene expression, and the result showed that it was positively influenced. As a result, it was observed that PEG/GG hydrogel promotes retinal regeneration compared to pure gellan gum. 3 wt% PEG/GG could be used as an alternative for retinal regeneration.