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Evaluation of polyherbal ayurvedic formulation ‘Peedantak Vati’ for anti-inflammatory and analgesic properties

Balkrishna, Acharya, Ranjan, Ravikant, Sakat, Sachin S., Sharma, Vinay K., Shukla, Ravikant, Joshi, Khemraj, Devkar, Raviraj, Sharma, Niti, Saklani, Sonia, Pathak, Prateek, Kumari, Pratima, Agarwal, Veena R.
Journal of ethnopharmacology 2019 v.235 pp. 361-374
analgesic effect, analgesics, anti-inflammatory activity, caffeic acid, carrageenan, cell lines, colchicine, curcumin, edema, formalin, herbs, high performance liquid chromatography, interleukin-6, macrophages, mice, minerals, models, monocytes, nitric oxide, nonsteroidal anti-inflammatory agents, pain, rats, rutin, tail, tandem mass spectrometry, traditional medicine, tumor necrosis factor-alpha, writhing test, India
Peedantak Vati (PV) is a polyherbal ayurvedic formulation, which is regularly prescribed by the ayurvedic practitioner for the inflammatory disorders and joints pain in India. It is composed of 23 different herbs and minerals, described in ayurvedic text for their anti-inflammatory and analgesic properties.To investigate anti-inflammatory and anti-nociceptive potential of ‘Peedantak Vati’ using in vitro and in vivo methods.In-vitro anti-inflammatory activity of PV was studied by estimating nitric oxide (NO) and LPS-induced pro-inflammatory cytokines IL-6 and TNF-α, using murine macrophage RAW264.7 and human monocyte THP-1 cell lines. PV's anti-inflammatory potential was studied in vivo using carrageenan-induced rat paw edema model. Similarly, anti-nociceptive property of PV was evaluated using hot plate, tail flick, formalin and writhing tests on CD-1 mice. Phytochemical profiling of hydro-alcoholic extract of PV was done using HPLC and HPTLC techniques to identify different marker compounds. These identified marker compounds were confirmed using LC-MS/MS analysis.In vitro results strongly suggest that, PV significantly (p < 0.001) inhibited NO release and LPS-stimulated pro-inflammatory cytokines IL-6 and TNF-α, in murine RAW264.7 and human THP-1 cells. Further, PV demonstrated significant (p < 0.05) anti-inflammatory activity at different time points after carrageenan injection with maximum effect at 2 h (40.4 ± 5.2% at 400 mg/kg). Similarly, PV significantly (p < 0.05) decreased nociceptive pain, studied using hot plate, tail flick, formalin and writhing tests. Moreover, HPLC and HPTLC methods were developed for the standardization of PV. Five marker phytocompounds viz. rutin, caffeic acid, colchicine, withaferin A and curcumin were identified and quantified by HPLC and HPTLC methods. The presence of these phytoconstituents was confirmed by LC-MS/MS analysis.The findings of the study strongly suggest that, the polyherbal ayurvedic formulation ‘Peedantak Vati’ possesses remarkable anti-inflammatory and analgesic property, providing potent alternative for currently available allopathic medicines such as non steroidal anti-inflammatory drugs (NSAIDs).