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Isolation of an antiviral compound from Tunisian olive twig cultivars

Author:
Leila, Abaza, Lamjed, Bouslama, Roudaina, Benzekri, Najla, Trabelsi, Taamalli, Ameni, Jellouli, Selim, Mokhtar, Zarrouk
Source:
Microbial pathogenesis 2019 v.128 pp. 245-249
ISSN:
0882-4010
Subject:
Enterovirus, Human herpesvirus 2, Olea europaea, active ingredients, antiviral agents, antiviral properties, branches, cultivars, ethyl acetate, gas chromatography-mass spectrometry, hexane, leaves, methanol, methylene chloride, olive oil, olives, plant extracts, thin layer chromatography, viruses
Abstract:
(1) Many studies demonstrated that olive oil and the leaves have several biological activities, but the works on olive twigs remain very limited. In this report, we investigated for anti-coxsackievirus B-3 (CVB-3) and anti-herpes virus type 2 (HSV-2) activities of olive twigs from two Tunisian varieties: Chemlali (CM) and Chétoui (CT). Extraction from the olive twigs was carried out using successively hexane, dichloromethane, ethyl acetate and methanol. The obtained extracts were evaluated for their anti-CVB-3 and anti-HSV-2 activities by MTT and plaque reduction assays, respectively. Only hexane extracts exhibited significant activity with a selectivity index of 6.32±0.67 and 5.24±0.82 for CM and CT, respectively. The active compound was isolated through bio-guided assays using Thin Layer Chromatography (TLC) and identified as 2,4-di-tert-butylphenol (2,4-DTBP) by gas chromatography coupled with mass spectrometry (GC-MS). This work is the first study that demonstrated an antiviral activity of both olive twigs and DTBP.
Agid:
6292183