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Clostridium strain selection for co-culture with Bacillus subtilis for butanol production from agave hydrolysates
- Oliva-Rodríguez, Alejandra G., Quintero, Julián, Medina-Morales, Miguel A., Morales-Martínez, Thelma K., Rodríguez-De la Garza, José A., Moreno-Dávila, Mayela, Aroca, Germán, Rios González, Leopoldo J.
- Bioresource technology 2019 v.275 pp. 410-415
- Agave lechuguilla, Bacillus subtilis, Clostridium acetobutylicum, anaerobic conditions, anaerobiosis, butanol, coculture, glucose, hydrolysates, nutrients, oxygen, reducing agents
- In this work, three Clostridium strains were tested for butanol production from Agave lechuguilla hydrolysates to select one for co-culturing. The agave hydrolysates medium was supplemented with nutrients and reducing agents to promote anaerobiosis. Clostridium acetobutylicum ATCC 824 had the highest butanol production (6.04 g/L) and was selected for further analyses. In the co-culture process, Bacillus subtilis CDBB 555 was used to deplete oxygen and achieve anaerobic conditions required for butanol production. The co-culture was prepared with C. acetobutylicum and B. subtilis without anaerobic pretreatment. Butanol production in co-culture from agave hydrolysates was compared with experiments using synthetic medium with glucose and a pure culture of C. acetobutylicum. The maximum butanol concentration obtained was 8.28 g/L in the co-cultured hydrolysate medium. Results obtained in the present work demonstrated that agave hydrolysates have the potential for butanol production using a co-culture of B. subtilis and C. acetobutylicum without anaerobic pretreatment.