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Sophorolipid produced from the new yeast strain Wickerhamiella domercqiae induces apoptosis in H7402 human liver cancer cells

Author:
Chen, Jing, Song, Xin, Zhang, Hui, Qu, Yin-bo, Miao, Jun-ying
Source:
Applied microbiology and biotechnology 2006 v.72 no.1 pp. 52-59
ISSN:
0175-7598
Subject:
apoptosis, caspase-3, cell proliferation, cytoplasm, humans, interphase, liver neoplasms, yeasts
Abstract:
The effects of sophorolipid on the growth and apoptosis of H7402 human liver cancer cells were investigated. By treatment with sophorolipid, a dose- and time-dependent inhibition of cell proliferation was observed. The cells developed many features of apoptosis, including condensation of chromatin, nuclear fragmentation, and appearance of apoptotic bodies, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling positive cells were stained dark brown. Sophorolipid treatment induced apoptosis in H7402 cells by blocking cell cycle at G1 phase and partly at S phase, activating caspase-3, and increasing Ca2+ concentration in cytoplasm. These findings may suggest a potential use of sophorolipid for liver cancer treatment.
Agid:
629311