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Suppression of riboflavin-sensitized singlet oxygen generation by l-ascorbic acid, 3-O-ethyl-l-ascorbic acid and Trolox

Author:
Shimizu, Ryohei, Yagi, Mikio, Kikuchi, Azusa
Source:
Journal of photochemistry and photobiology 2019 v.191 pp. 116-122
ISSN:
1011-1344
Subject:
absorption, ascorbic acid, buffers, dissolved oxygen, eyes, fluorescence, pH, phosphates, phosphorescence, photosensitizing agents, riboflavin, singlet oxygen, skin (animal), vitamin E, water solubility
Abstract:
Riboflavin (RF), a water-soluble vitamin B2, is an endogenous singlet oxygen photosensitizer in human skin and eye. Time profiles of the near-infrared phosphorescence of singlet oxygen generated by RF have been measured in the absence and presence of l-ascorbic acid (AA, vitamin C), 3-O-ethyl-l-ascorbic acid (3-EtAA) and Trolox (TX, a water-soluble analogue of vitamin E) in phosphate buffer (pH 6.8). These substances suppress the RF-photosensitized singlet oxygen generation. For example, the quantum yield of singlet oxygen generation is decreased to a third by adding 0.4 mmol dm−3 AA or TX (the concentration of dissolved oxygen in air-saturated water is 0.27 mmol dm−3). AA and TX are more efficient suppressors of RF-photosensitized singlet oxygen generation than 3-EtAA. The bimolecular rate constants for quenching of the excited singlet and triplet states of RF by AA, 3-EtAA and TX have been determined through measurements of fluorescence and transient absorption. These measurements suggest that the observed suppression is due to the quenching of the excited singlet and triplet states of RF by AA, 3-EtAA and TX. The bimolecular rate constants for quenching of singlet oxygen by AA, 3-EtAA and TX were determined to be 1.8 × 108, 0.27 × 108, and 4.4 × 108 mol−1 dm3 s−1, respectively.
Agid:
6293190